mouse monoclonal anti ha (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc mouse monoclonal anti ha

CHIKV uptake in Huh7 cells is related to the AGPAT1 protein levels. ( A ) Huh7 cells were transfected with siRNA to AGPAT1 (siAGPAT1) or a control non-targeting siRNA (siNT) and incubated for 48 h at 37°C. Western blotting showed a ~60% reduction in the AGPAT1 levels in the siAGPAT1-treated cells. ( B ) The siRNA-treated cells were incubated 48 h post-transfection (pt) with CHIKV (MOI 1) for 1 h on ice for the virus uptake assay. The relative CHIKV RNA levels are shown (left panel), where the CHIKV RNA level in the siNT-transfected cells at 1h post-infection (pi) was taken as 1. The viral titers determined by plaque assay are shown in the right panel. The data from four biological replicates and two technical replicates are shown. ( C ) Huh7 cells were transfected with the pAGPAT1 plasmid expressing the HA-tagged AGPAT1 or the empty vector pcDNA5. The cells were harvested at 24 and 48 h later. The cell lysates were western blotted with <t>monoclonal</t> <t>anti-HA</t> antibody to detect the expression of the HA-tagged AGPAT1. GAPDH was used as the loading control. ( D ) Huh7 cells were transfected with pAGPAT1-HA or pcDNA5, and 48 h later, they were incubated with CHIKV (MOI 5) for the virus binding assay. The relative CHIKV RNA levels are presented (left panel), where the CHIKV RNA level at 1 h pi was taken as 1. The right panel has the virus titers. The data from three biological replicates and two technical replicates are shown. ** P < 0.01, *** P < 0.001.

Mouse Monoclonal Anti Ha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1074 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti+ha+tag/HA-Tag+Mouse+mAb/pmc13011415-305-43-47
Average 96 stars, based on 1074 article reviews

mouse monoclonal anti ha - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "AGPAT1 is a novel Chikungunya virus receptor on human cells"

Article Title: AGPAT1 is a novel Chikungunya virus receptor on human cells

Journal: Journal of Virology

doi: 10.1128/jvi.01733-25

Figure Legend Snippet: CHIKV uptake in Huh7 cells is related to the AGPAT1 protein levels. ( A ) Huh7 cells were transfected with siRNA to AGPAT1 (siAGPAT1) or a control non-targeting siRNA (siNT) and incubated for 48 h at 37°C. Western blotting showed a ~60% reduction in the AGPAT1 levels in the siAGPAT1-treated cells. ( B ) The siRNA-treated cells were incubated 48 h post-transfection (pt) with CHIKV (MOI 1) for 1 h on ice for the virus uptake assay. The relative CHIKV RNA levels are shown (left panel), where the CHIKV RNA level in the siNT-transfected cells at 1h post-infection (pi) was taken as 1. The viral titers determined by plaque assay are shown in the right panel. The data from four biological replicates and two technical replicates are shown. ( C ) Huh7 cells were transfected with the pAGPAT1 plasmid expressing the HA-tagged AGPAT1 or the empty vector pcDNA5. The cells were harvested at 24 and 48 h later. The cell lysates were western blotted with monoclonal anti-HA antibody to detect the expression of the HA-tagged AGPAT1. GAPDH was used as the loading control. ( D ) Huh7 cells were transfected with pAGPAT1-HA or pcDNA5, and 48 h later, they were incubated with CHIKV (MOI 5) for the virus binding assay. The relative CHIKV RNA levels are presented (left panel), where the CHIKV RNA level at 1 h pi was taken as 1. The right panel has the virus titers. The data from three biological replicates and two technical replicates are shown. ** P < 0.01, *** P < 0.001.

Techniques Used: Transfection, Control, Incubation, Western Blot, Virus, Infection, Plaque Assay, Plasmid Preparation, Expressing, Binding Assay

CHIKV binding and uptake in Huh7 cells ectopically expressing the AGPAT1 mutant proteins. ( A ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The non-permeabilized cells were blocked and then stained with the rabbit monoclonal anti-HA antibody, followed by fixing and staining with anti-rabbit Alexa Fluor-568 antibody. The cells were imaged to examine the expression of the ectopically produced AGPAT1-HA or its mutants on the plasma membrane. ( B ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The cells were washed at 48 h pt and incubated with CHIKV (MOI 50) on ice for 1 h and stained with CHIKV anti-E2 antibody to study the virus binding by microscopy. ( C ) The MIFD (25 ROI) data of the transfected Huh7 cells stained as above for the CHIKV binding are presented from three biological replicates. ( D ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The cells were incubated 48 h later with CHIKV (MOI 3) for the virus uptake assay. The relative CHIKV RNA levels are presented, where the CHIKV RNA level in cells transfected with pcDNA5 was taken as 1. The data from five biological replicates and two technical replicates are shown. ( E ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The cells were infected 48 h later with CHIKV (MOI 3) and incubated at 37°C. The virus titers in the culture supernatant at 6 h pi are presented. The data from four biological replicates and two technical replicates are shown. The statistical analysis on data shown as mean±SD was done by the one-way ANOVA with Dunnett’s multiple comparisons test, considering the pcDNA5-transfected cells as the control. *** P < 0.001, or ns = not significant.

Figure Legend Snippet: CHIKV binding and uptake in Huh7 cells ectopically expressing the AGPAT1 mutant proteins. ( A ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The non-permeabilized cells were blocked and then stained with the rabbit monoclonal anti-HA antibody, followed by fixing and staining with anti-rabbit Alexa Fluor-568 antibody. The cells were imaged to examine the expression of the ectopically produced AGPAT1-HA or its mutants on the plasma membrane. ( B ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The cells were washed at 48 h pt and incubated with CHIKV (MOI 50) on ice for 1 h and stained with CHIKV anti-E2 antibody to study the virus binding by microscopy. ( C ) The MIFD (25 ROI) data of the transfected Huh7 cells stained as above for the CHIKV binding are presented from three biological replicates. ( D ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The cells were incubated 48 h later with CHIKV (MOI 3) for the virus uptake assay. The relative CHIKV RNA levels are presented, where the CHIKV RNA level in cells transfected with pcDNA5 was taken as 1. The data from five biological replicates and two technical replicates are shown. ( E ) Huh7 cells were transfected with the plasmids expressing AGPAT1-HA or the mutant proteins, or the empty vector pcDNA5. The cells were infected 48 h later with CHIKV (MOI 3) and incubated at 37°C. The virus titers in the culture supernatant at 6 h pi are presented. The data from four biological replicates and two technical replicates are shown. The statistical analysis on data shown as mean±SD was done by the one-way ANOVA with Dunnett’s multiple comparisons test, considering the pcDNA5-transfected cells as the control. *** P < 0.001, or ns = not significant.

Techniques Used: Binding Assay, Expressing, Mutagenesis, Transfection, Plasmid Preparation, Staining, Produced, Clinical Proteomics, Membrane, Incubation, Virus, Microscopy, Infection, Control

Western Blot:

Article Title: Cholesterol pathway inhibition induces TGFβ signaling to promote basal differentiation in pancreatic cancer.
Article Snippet: Rabbit monoclonal anti-beta-actin (clone 13E5) (for WB 1:1000) , Cell Signaling , Cat#4970; RRID:AB_2223172. .. Mouse monoclonal anti-HA-tag (clone 6E2) (for WB 1:1000) , Cell Signaling , Cat#2367S; RRID:AB_10691311. .. Rabbit polyclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (for WB 1:1000) , Cell Signaling , Cat#9101; RRID:AB_331646.

Article Title: Structural basis of mitochondrial protein import by the TIM23 complex.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse monoclonal anti-Myc-tag (Invitrogen #13-2500, 1:1000), mouse monoclonal anti-HA-tag (Invitrogen #26183, 1:5000), rabbit monoclonal anti-HA-tag (Cell Signaling #3724, 1:1000), mouse monoclonal anti-His-tag (Invitrogen #MA1-21315, 1:1000), mouse monoclonal anti-Strep-tag (Genscript #A01732, 1:2000), mouse monoclonal anti-GFP-tag (Roche #11814460001, 1:1000), rabbit monoclonal anti-GFP-tag (Genscript #A01388, 1:1000), mouse monoclonal anti-Tim44 (Clone #6; generated in this study for cryo-EM analysis), mouse polyclonal anti-Tim44 (Clone #23; generated in this study for immunoblotting, 1:100), rabbit polyclonal anti-Tom22 (gift from N. Pfanner, homemade stock, 1:1000), rabbit polyclonal anti-Tom40 (gift from N. Pfanner, homemade stock, 1:500), rabbit polyclonal anti-Pgk1 (gift from J. Thorner, homemade stock, 1:1000), rabbit polyclonal anti-Pam16 (gift from E. Craig, homemade stock, 1:2500), and rabbit polyclonal anti-Pam18 (gift from E. Craig, homemade stock, 1:1000). .. Secondary antibodies conjugated to HRP used in this study were goat anti-rabbit (Thermo #31460, 1:10,000), goat anti-mouse (Thermo #31430, 1:10,000), and mouse anti-rabbit (Genscript #A01827, 1:10,000).

Chromatin Immunoprecipitation:

Flow Cytometry:

Magnetic Resonance Imaging:

Generated:

Immunofluorescence:

Article Title: Endoglin Wild Type and Variants Associated With Hereditary Hemorrhagic Telangiectasia Type 1 Undergo Distinct Cellular Degradation Pathways
Article Snippet: Antibodies for Western blot analysis: Rabbit monoclonal anti-HA-tag (Cell Signaling Technology, at 1:1,000 dilution), Rabbit monoclonal anti-SYVN1 (Cell Signaling Technology, at 1:1,000 dilution), mouse monoclonal anti-GAPDH (Abcam, at 1:2,500 dilution) rabbit monoclonal Anti-HA (Cell Signaling, at 1:1,000 dilution), mouse monoclonal anti-RFP (Thermo Fisher Scientific, at 1:1,000 dilution), rabbit anti- Endoglin P3D1 (Santa Cruz Biotechnology, at 1:200 dilution), anti-Mouse IgG Peroxidase antibody (Sigma Aldrich, at 1:40,000 dilution), and anti-Rabbit IgG Peroxidase antibody (Sigma Aldrich, at 1:30,000 dilution). .. Antibodies for immunofluorescence: mouse monoclonal anti-HA-tag (Cell signaling Technology, at 1: 200 dilution), rabbit polyclonal anti-calnexin (Santa Cruz Biotechnology, at 1 : 200 dilution), rabbit anti-Histone-H3 (Cell Signaling Technology, at 1:1,000 dilution), mouse monoclonal anti-HA (Cell Signaling Technology, at 1: 200 dilution), Alexa Fluor 568-goat anti-mouse IgG (Molecular Probes, at1:200 dilution), and Alexa Fluor 488-goat anti rabbit IgG (Molecular Probes, at 1:200dilution). ..

Article Title: Endoglin mutants retained in the endoplasmic reticulum exacerbate loss of function in hereditary hemorrhagic telangiectasia type 1 (HHT1) by exerting dominant negative effects on the wild type allele.
Article Snippet: Antibodies used for Western blot analysis were the following: rabbit monoclonal anti-GAPDH (Abcam, at 1:2500 dilution), rabbit monoclonal anti-HA (Cell Signaling, at 1:1000 dilution), rabbit monoclonal antiCalnexin (Cell Signaling, at 1:1000 dilution) and mouse monoclonal anti-Myc (Cell Signaling Technology, at 1:500 dilution). .. Antibodies used for immunofluorescence: mouse monoclonal anti-HA-tag (Cell Signaling Technology, at 1: 200 dilution), rabbit monoclonal anti-HA (Cell Signaling, at 1:200 dilution), rabbit polyclonal anti-Calnexin (Santa Cruz Biotechnology, at 1: 200 dilution), rabbit anti-Na+K+ ATPase (Abcam, at 1:500 dilution), Alexa Fluor 488-goat anti rabbit IgG (Molecular Probes, at 1:000 dilution), Alexa Fluor 350 chicken anti-goat (Invitrogen, at 1:1000 dilution), goat polyclonal anti-Calnexin (Santa Cruz Biotechnology, at 1:50) Alexa Fluor 488 donkey anti mouse (Invitrogen, at 1:1000 dilution), Alexa Fluor 568 donkey anti rabbit (Invitrogen, at 1:1000 dilution). ..

Article Title: Cellular and functional evaluation of LDLR missense variants reported in hypercholesterolemic patients demonstrates their hypomorphic impacts on trafficking and LDL internalization
Article Snippet: Sanger DNA sequencing was performed using the dideoxy Sanger method by automated fluorescence sequencing on the ABI 3130xl Genetic Analyzer (Applied Biosystems, Waltham, MA, United States) to confirm the generation of intended amino acid changes within the LDLR cDNA. .. For immunofluorescence, we used mouse monoclonal anti-HA-tag [(1:200; Cell Signaling Technologies (CST; Danvers, MA, United States)], rabbit polyclonal anti-calnexin (CANX 1:50; Santa Cruz Biotechnology, Dallas, TX, United States), Alexa Fluor 488-goat anti-rabbit IgG (1:200; Molecular Probes, Eugene, OR, United States), Alexa Fluor 555-goat anti-mouse IgG (1:200; Molecular Probes). .. For Western blotting, we used mouse monoclonal anti-HA [(1:4000; Cell Signaling Technologies (CST; Danvers, MA, United States)], mouse monoclonal anti-B actin (1:1000; Santa Cruz Biotechnology, Dallas, TX, United States).

Immunodetection:

Article Title: Late stage melanoma is hallmarked by low NLGN4X expression leading to HIF1A accumulation
Article Snippet: .. The following primary antibodies were applied for immunodetection as follows: rabbit polyclonal anti-NLGN4X (Sigma-Aldrich, HPA001651, 1:1000, previously used by Davidson et al. [ ]), rabbit polyclonal anti-VBP1 (Sigma-Aldrich, HPA023230, 1:1000), mouse monoclonal anti-HIF1A (Santa Cruz, Dallas, TX, sc53546, 1:1000), rabbit monoclonal anti-TXNIP (Abcam, Cambridge, UK, EPR14774, 1:1000), rabbit monoclonal anti-pH2AX (Cell Signalling, 9718, 1:1000), mouse monoclonal anti-HA-Tag (Cell Signalling, 2367, 1:1000). .. On the following day, membranes were incubated with HRP-linked heavy and light chain antibodies (Anti-Mouse IgG: Thermo Fisher Scientific, Waltham, MA, 31430, 1:5000, Anti-Rabbit IgG: Thermo Fisher Scientific, 31460, 1:5000).

Article Title: Late stage melanoma is hallmarked by low NLGN4X expression leading to HIF1A accumulation.
Article Snippet: .. The following primary antibodies were applied for immunodetection as follows: rabbit polyclonal anti-NLGN4X (Sigma-Aldrich, HPA001651, 1:1000, previously used by Davidson et al. [15]), rabbit polyclonal anti-VBP1 (Sigma-Aldrich, HPA023230, 1:1000), mouse monoclonal anti-HIF1A (Santa Cruz, Dallas, TX, sc53546, 1:1000), rabbit monoclonal anti-TXNIP (Abcam, Cambridge, UK, EPR14774, 1:1000), rabbit monoclonal anti-pH2AX (Cell Signalling, 9718, 1:1000), mouse monoclonal anti-HA-Tag (Cell Signalling, 2367, 1:1000). .. On the following day, membranes were incubated with HRP-linked heavy and light chain antibodies (Anti-Mouse IgG: Thermo Fisher Scientific, Waltham, MA, 31430, 1:5000, Anti-Rabbit IgG: Thermo Fisher Scientific, 31460, 1:5000).

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